FIGURE

Fig. S5

ID

ZDB-FIG-181120-20

Publication

Otten et al., 2018 - Systematic pharmacological screens uncover novel pathways involved in cerebral cavernous malformations

Other Figures

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Fig. S5

Indirubin-3-monoxime (IR3mo) rescues the CCM phenotype in krit1^ty219c mutant zebrafish and CCM2- and CCM3-depleted HUVECs.

Treatment with 5 lM IR3mo rescues the embryonic zebrafish krit1ty219c mutant ballooning heart phenotype. Shown are images of confocal z-scan projections of wild-type (WT) (A, C) and krit1ty219 mutant (B, D) zebrafish embryonic hearts at 48 hpf expressing the endothelial reporter Tg(kdrl:GFP)s843 (green) and counterstained for ACTIN (red). Scale bar is 50 lm.

Treatment with 10 lM IR3mo for 48 h rescued the CCM phenotype in CCM2- or CCM3-depleted HUVECs. Shown are confocal images of HUVECs with labeled VE-cadherin (green) and F-ACTIN (red). Control siRNA-silenced (E, F), CCM2 siRNA-silenced (G, H), or CCM3 siRNA-silenced (I, J) HUVECs untreated (E, G, I) or treated (F, H, J) with IR3mo. Scale bar is 10 lm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	krit1^{ty219c/ty219c}; s843Tg
Condition:	chemical treatment by environment: indirubin-3'-monoxime
Observed In:	heart
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.