Fig. 1

Immunoreactivity of anti-CNGA2a mAb in larval and adult zebrafish. (A) Phylogram of the CNG channels protein sequences. Comparison of the CNGA2 protein homologues from zebrafish, human, and mouse species is enclosed in the blue box. The scale bar indicates 30% amino acid residues substitution. (B,C) Confocal images showing representative labeling of the OXT perikarya, neuronal projections, and neurohypophyseal axonal termini with anti-CNGA2a mAb (magenta) and anti-OXT Ab (gray scale) in the context of the EGFP-positive OXT-ergic population in oxt:egfp reporter (green). Immunohistochemical analysis show colocalization of EGFP+, OXT+, and CNGA2a+ moieties in the cell bodies, axons, and nerve termini in the neurohypophysis of 6-day-old larva (n = 30/30) (B) and dissected brain and pituitary from 3-month-old adult zebrafish (n = 3/3) (C). Scale bars: 10 μm.