FIGURE

Fig. 3

ID

ZDB-FIG-181101-3

Publication

Willoughby et al., 2012 - Generation of a genetically encoded marker of rod photoreceptor outer segment growth and renewal

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Fig. 3

Expression of HA-mCherry^TM in photoreceptors after heat-shock at 3 dpf.

(A–C) At 5 hpHS, a single confocal z-section of a Tg(hsp70:HA-mCherry^TM) photoreceptor layer labeled with anti-HA antibody (red), anti-Rhodopsin antibody (blue) and GFP-expressing rods (green) shows membrane expression of HA-mCherry^TM in rods and neighboring cones, and a stripe of HA-mCherry^TM at the base of rod outer segments (arrows, A, B). (D–F) At 1 dpHS, a single confocal z-section of a Tg(hsp70:HA-mCherry^TM) photoreceptor layer labeled with anti-HA antibody (red), anti-Rhodopsin antibody (blue) and GFP-expressing rods (green) shows that membranous HA-mCherry^TM labeling in the cell body and inner segment has largely disappeared and a stripe of HA-mCherry^TM in a rod outer segment has been displaced distally (double arrows, D–F). Continuous HA-mCherry^TM labeling largely fills cone outer segments (arrowheads, D–F). Scale bar: A–F, 5 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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