FIGURE

Fig. 5

ID

ZDB-FIG-181011-31

Publication

Zelinka et al., 2018 - Targeted disruption of the endogenous zebrafish rhodopsin locus as models of rapid rod photoreceptor degeneration.

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

rho^fl7 and rho^fl9 lead to increased TUNEL in adults. Retinal sections of WT (A,D,G) or heterozygous rho^fl7^/+ (B,E,H) and rho^fl9^/+ (C,F,I) adult zebrafish immunolabeled with antibodies to rods (4C12, green, A-C), Rho (1D1, red, D-F), or red/green cones (Arr3a/Zpr-1, red, G-I) and stained with TUNEL (red, A-C). All sections were counterstained with DAPI (blue). Immunolabeling for rods reveals fewer, less regularly arranged cells in the mutants compared to the WT. TUNEL-positive nuclei (arrows), positioned along the proximal region of the ONL, are only observed in the mutant retinas (A-C). In WT retinas, Rho immunolabeling is restricted to the ROS at the top of the panel D. Immunolabeling in rho^fl7^/+ and rho^fl9^/+ adults is localized to the cell bodies, and no outer segments are evident (E-F). Immunolabeling for red and green cones was indistinguishable across the samples (G-H). Abbreviations: ganglion cell layer, GCL; inner nuclear layer, INL; outer nuclear layer, ONL.

Expression Data

Gene:	rho
Antibodies:	Ab-4C12 Ab1-rho
Fish:	rho^fl7/+ rho^fl9/+
Anatomical Term:	retinal rod cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rho^fl7/+ rho^fl9/+
Observed In:	retina apoptotic process retinal rod cell retinal rod cell photoreceptor outer segment
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.