Fig. 3

Rod but not cone photoreceptors are reduced in rho^djh503+/− mutants. (A) The 1d1 antibody (magenta), which recognizes Rhodopsin, labels the outer segments of wildtype rod cells. The labeling pattern is similar in both wildtype and mutants at 3 dpf. At 6 dpf, 1d1 antibody labeling is restricted to the proliferating marginal zone in rho^djh503+/− mutants (*), but remains throughout the ONL in wildtype retinas. The white boxed region in the 6 dpf images are enlarged in the panels to the right showing that the 1d1 antibody labels YFP-expressing rod cells (green, arrows). (B) The zpr1 (magenta) antibody labeled cone cells do not show an obvious difference between wildtype and mutant retinas at 4 and 7 dpf. The boxed region in the 7 dpf images is enlarged to the right showing no overlay between zpr1 antibody staining and YFP-expressing rod cells (green). DAPI (blue) was used to stain cell nuclei. Arr3a, Arrestin 3a; DAPI, 4′,6-diamidino-2-phenylindole; dpf, days post-fertilization; ONL, outer nuclear layer; rho, rhodopsin; Rho, Rhodopsin; YFP, yellow fluorescent protein.