FIGURE

Fig. 2

ID

ZDB-FIG-180918-12

Publication

Rosowski et al., 2018 - Macrophages inhibit Aspergillus fumigatus germination and neutrophil-mediated fungal killing

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Inflammatory activation is required for clearance of a CEA10-derived strain.

A, B. NF-κB RE:EGFP larvae were infected with non-fluorescent TJW55.2 (Af293) or CEA17 KU80Δ (CEA10) spores and imaged 2 dpi. Representative images (A) and quantification from three pooled experiments (B) are shown. Scale bar represents 50 μm. CEA10 n = 22; Af293 n = 26. C. Larvae were treated with dexamethasone (DEX) or ethanol (EtOH) vehicle control directly after infection with TFYL81.5 (Af293) or TFYL49.1 (CEA10), and larval survival was monitored. Average injection CFUs: Af293 = 34, CEA10 = 33. D-F. myd88^-/- or control larvae were infected with TBK1.1 (Af293) or TFYL49.1 (CEA10) and larval survival and/or fungal burden was monitored. Fungal burden was determined by CFU platings from single homogenized larvae. Asterisks represent significance between myd88^-/- and control larvae, # represent significance compared to day 0. Average injection CFUs: D, F: Af293 = 27, CEA10 = 32; E: Af293 = 66. For all analyses, data represent 3 pooled replicates. For CFU experiments, CFUs from 24 larvae (8 larvae per replicate) per condition per day were measured. Intensity values and CFU analyses represent lsmeans ± SEM from three pooled experiments, P values calculated by ANOVA. P values for survival analyses calculated by Cox proportional hazard regression analysis. See also S2 Fig.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.