Fig. S1
Organization of adult DRG peripheral axons, Related to Figure 1 (A) Lateral view of the adult trunk. Note the absence of the somatosensory axon reporter [Tg(p2rx3a>mCherry)] along the posterior lateral line nerve (pLLn), a sensory nerve that innervates neuromasts along the trunk. Arrows, superficial axon bundles. Dashed line, scale margin. (B-C) Maximum intensity projections through the basal epidermis of scales isolated from indicated transgenic lines that mark sensory neurons. Arrows, superficial axon bundles. (D) Maximum intensity projections through the basal epidermis of isolated scales immunostained as indicated. (E) Transverse vibratome section through the adult mid-trunk. Arrows, DRG cell bodies. Yellow arrowheads, DRG peripheral axons between myosepta. Dashed box, area of magnification. Doubleheaded arrows, DRG central axons within the dorsal spinal cord (sc). Dashed lines, spinal cord boundary. Transgenes: (A) somatosensory axons [Tg(p2rx3a>mCherry)] and myelinating Schwann cells [Tg(cldnk:GAL4);Tg(14xUAS:MA-GFP)] (Münzel et al., 2012); (B) Tg(Tru.Gap43:EGFP) (Udvadia, 2008); (C) TgBAC(trpa1b:EGFP) (Pan et al., 2012); (E) Tg(p2rx3a>mCherry). Staining: (D) axons (zn-12) (Metcalfe et al., 1990), axons (acTubulin), and nuclei (DAPI); (E) nuclei (DAPI). Scale bars, 250 μm (A,E), 25 μm (B-D) and 100 μm (E’’). |
Reprinted from Developmental Cell, 46(3), Rasmussen, J.P., Vo, N.T., Sagasti, A., Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization, 344-359.e4, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell