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Disruption of glycine signaling causes an early and transient neural cell death during embryonic development. (A) Lateral views of zebrafish embryos stained by AO. live embryos injected by Ctrl-MO, Glr-MO, and p53-Glr-MO were stained by AO and imaged at 20, 24, and 48 hpf, higher magnification of cell death at spinal cord regions were highlighted in dotted boxed area. (B) Lateral view of zebrafish embryos at 20 and 24 hpf embryos injected by Glr-MO or Ctlr-MO and labeled by immunostaining for aCas3 (white dots), dotted box in the left and bottom showed a magnification of positives aCase3 in the brain and spinal cord. (C) Cell death quantification during zebrafish embryos development. AO positive cells at the spinal cord were quantified and compared in each condition, uninjected, Ctrl-MO, Glr-MO and p53-Glr-MO injected embryos at four-time points, 14, 20, 24, and 48 hpf. One-way ANOVA statistical analysis was performed (n = 15, ****p < 0.0001).
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