Fig. 7

Less neutrophils are recruited at the infection in Tg(hsp:Myc-npsn) embryos. (a) The infection site of the zebrafish muscle (the red arrow). DsRed⁺ E. coli were injected subcutaneously over one somite into WT and Tg(hsp:Myc-npsn) embryos with Tg(mpx:eGFP) background, and images were captured at 0.5, 3 and 24 hpi. All images are maximum-intensity projection at an interval of 2 μm. White triangles indicated neutrophils with phagosomes. (b) Quantification of recruited mpx:eGFP⁺ neutrophil numbers (green bar) and phagocytosing neutrophil numbers (red net bar) in the infection site at each time point in bacterial injected WT and Tg(hsp:Myc-npsn) embryos. Average numbers with means in WT and Tg(hsp:Myc-npsn) groups at 0.5, 3, 24 hpi (green bar: 41.0 ± 2.0 versus 32.8 ± 1.5; 61.2 ± 3.1 versus 48.4 ± 3.0; 47.8 ± 4.5 versus 33.0 ± 3.7); (red net bar: 33.0 ± 3.3 versus 28.2 ± 81.3; 57.6 ± 3.0 versus 44.3 ± 3.7; 2.2 ± 0.6 versus 2.4 ± 0.7). (Mean±s.e.m., n ≥ 6 in each group, triplicated). Statistical significance was determined using the two-way ANOVA with Bonferroni's multiple comparisons adjustment. *p < 0.05. **p < 0.01. ***p < 0.001. n.s., not significant. (c) Bacterial burden of embryos at 24 hpi in Tg(hsp:Myc-npsn) embryos and WT controls. (Mean±s.e.m., n = 50 in each group, triplicated). Statistical significance was determined using the unpaired t-test. *p < 0.05.