Zebrafish hhex mutants lack lymphatic precursors. a?d Whole-mount views of 36?hpf hhex+/+ and hhex?/? embryos immunostained for Prox1 (arrowheads point to Prox1+ endothelial cells in the PCV). e Quantification of the number of Prox1+ endothelial cells across three somites (from two field of view per embryo) in hhex+/? (n?=?8) and hhex?/? (n?=?6). f?i Whole-mount views of 36?hpf Tg(prox1a:TagRFP); hhex+/+ and hhex?/? embryos. hhex mutants exhibit a strong decrease in the number of RFP+ cells in the PCV compared to hhex+/+ (arrowheads point to RFP+ endothelial cells in the PCV). j Quantification of the number of RFP+ endothelial cells across three somites (from two field of view per embryo) in hhex+/? (n?=?10) and hhex?/? (n?=?4). Values represent means?±?s.e.m. ***P???0.001, and **P???0.01 by t-test. Scale bars: 50??m
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