FIGURE

Fig. 4

ID

ZDB-FIG-180827-16

Publication

Ferrero et al., 2018 - Embryonic Microglia Derive from Primitive Macrophages and Are Replaced by cmyb-Dependent Definitive Microglia in Zebrafish

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Fig. 4

Kinetics of Adult MG Emergence during Zebrafish Development

(A) Scheme of the transgenic lines used to discriminate pMF-derived embryonic MG from endothelial-derived adult MG.

(B) GFP (left panels) and DsRed (middle panels) immunostaining performed on brain sections from Tg(kdrl:Cre; ßactin:Switch-DsRed; mpeg1:GFP) triple transgenics at the indicated developmental stage, showing the replacement of GFP⁺ DsRed⁻ embryonic MG by GFP⁺ DsRed⁺ cells in the brain parenchyma. The right panels show a merge of both fluorescent channels. Scale bar: 50 μm.

(C) Quantification of DsRed⁺ MG in the brain parenchyma during zebrafish development, determined as percent recombination among the whole GFP⁺ MG population. Error bars represent mean ± SEM of pooled data from two experiments (n = 3). MG, microglia.

Expression Data

Genes:	DsRedx EGFP
Fish:	gl22Tg; s898Tg; sd5Tg
Anatomical Term:	brain microglial cell
Stage Range:	Days 7-13 to Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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