FIGURE

Fig. 7

ID

ZDB-FIG-180824-26

Publication

Collins et al., 2018 - Pitx2c orchestrates embryonic axis extension via mesendodermal cell migration

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Fig. 7

Pitx2c modulates the chemokine and ECM-integrin signaling axis.

Analysis of the chemokine signaling axis and ECM factors that control endodermal migration. (a–c) qPCR analysis of cxcl12b (a), cxcr4a (b), and itgb1b (c) mRNA levels in wild-type, MZpitx2c mutant, and pitx2c GOF embryos at 80% epiboly. (d–i) Fibronectin staining in wild-type (d–f) and MZpitx2c mutant (g–i) embryos in the Tg(sox17:GFP) background to visualize endodermal cells. While fibronectin deposition appears fibrillar by the two ss in wild-type embryos (e), a less dense network is observed in MZpitx2c mutants (h). ‘n’ refers to the number of embryos with the expression pattern shown over the total number of embryos analyzed. *p<0.05, **p<0.01 by unpaired t-test. Graphs indicate the average fold change ± SEM from at least three biological replicates analyzed in duplicates. Scale bars, 20 μm.

Expression Data

Genes:	cxcl12b cxcr4a GFP itgb1b
Antibody:	Ab2-fn
Fish:	WT pitx2^ups6/ups6 s870Tg pitx2^ups6/ups6; s870Tg
Anatomical Terms:	endoderm extracellular matrix endodermal cell whole organism
Stage Range:	75%-epiboly to 1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pitx2^ups6/ups6 pitx2^ups6/ups6; s870Tg
Observed In:	endoderm extracellular matrix extracellular matrix organization fibronectin fibril organization whole organism
Stage Range:	75%-epiboly to 1-4 somites

Phenotype Detail

Acknowledgments

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