FIGURE

Fig. 7

ID

ZDB-FIG-180727-33

Publication

Eno et al., 2018 - Slow calcium waves mediate furrow microtubule reorganization and germ plasm compaction in the early zebrafish embryo

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Fig. 7

FMA distal enrichment correlates with SCW directionality. (A-B′) Calcium levels in single Tg[βactin2:GCaMP6s] transgenic embryos during furrow progression, with plotted profiles (A′,B′) corresponding to boxed areas. Wild-type embryos (A′), but not nebel mutants (B′), show increasing distal calcium levels. (C-D′) Microtubules in EMTB::EGFP transgenic embryos during furrow progression (C,D), with plotted profiles (C′,D′). Wild-type embryos (C′), but not nebel mutants (D′), show distal enrichment. Time points T1, T2 and T3 correspond, respectively, to frames within one-tenth of the total sequence of acquired images spanning a furrow formation cycle at the beginning, middle and end of that cycle. See Fig. S4 for additional profiles.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nebel^t23/+; hm1Tg (AB) nebel^t23/+; stl351Tg (AB)
Observed In:	blastomere microtubule cytoskeleton organization blastomere release of sequestered calcium ion into cytosol
Stage Range:	2-cell to 4-cell

Phenotype Detail

Acknowledgments

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