FIGURE

Fig. 5

ID

ZDB-FIG-180727-24

Publication

Gao et al., 2018 - The Midline Axon Crossing Decision Is Regulated through an Activity-Dependent Mechanism by the NMDA Receptor

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Fig. 5

arxa levels are regulated by activity and the NMDAR. A–A’’, foxP2.A.2:egfp-caax and arxa are coexpressed in early development as the TCPT axons extend toward the midline. Sections, rostral top, of arxa in situ expression and Tg(foxP2.A.2:egfp-caax) α-GFP immunohistochemistry. Red boxed inset shows sense probe control for arxa. B, arxa expression is reduced following blockade (APV) or knock-down (CRISPR) of NMDAR or inhibiting activity (tricaine). qRT-PCR at 48 hpf, experimental triplicates; n > 10 embryos each group. Relative log2 fold change calculated relative to wild-type, elf1a standard, SD for each in parenthesis, p values adjusted using Benjamini–Hochberg false discovery rate; **p < 0.01; error bars, SEM.

Expression Data

Genes:	arxa EGFP
Fish:	WT zc69Tg WT + CRISPR1-grin1a + CRISPR1-grin1b
Conditions:	chemical treatment by environment: NMDA receptor antagonist chemical treatment by environment: tricaine
Knockdown Reagents:	CRISPR1-grin1a CRISPR1-grin1b
Anatomical Terms:	head tract of the commissure of the caudal tuberculum neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + CRISPR1-grin1a + CRISPR1-grin1b
Conditions:	chemical treatment by environment: NMDA receptor antagonist chemical treatment by environment: tricaine
Knockdown Reagents:	CRISPR1-grin1a CRISPR1-grin1b
Observed In:	head
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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