FIGURE

Fig. 3

ID

ZDB-FIG-180713-1

Publication

Li et al., 2018 - The dual role of cGMP in oocyte maturation of zebrafish

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Fig. 3

Regulation of NO by hCG in ovarian follicles of zebrafish.

(A) The oocyte maturation could be dynamically induced by injection of hCG (15 IU/fish). (B) Gross morphology of ovaries of FG stage intact follicles, which were manually separated into two comportments, follicular cells and denuded oocytes. (C) PI staining of intact follicles (upper panel) and denuded oocytes (lower panel). (D) After injection of hCG (15 IU/fish), the NO concentration was assessed in intact follicles (a), denuded oocytes (b) and follicular cells (c) at different times. (E) Morphology of primary cultured zebrafish ovarian follicular cells after 6 days of incubation. The two types of epithelial cells present in the culture with distinct morphologies: G, granulosa cells; T, theca cells. (F) The NO level was assessed in primary culture follicular cells by treatment with hCG (20 IU/mL) for 2 h. Each value represents the mean value ± SEM of quintuplicate assays from three independent experiments (*, P < 0.05 vs. control).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.