FIGURE

Fig. 5

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ZDB-FIG-180711-9

Publication

Nikaido et al., 2018 - Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene

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Fig. 5

Immunostaining of 5-HT or Nos1 with GFP in the gut of Tg(chata:GGFF2) larvae. Some of the GFP + cells expressed either 5-HT or Nos1. Double staining of GFP and 5-HT (A?D) or, GFP and Nos1 (E?H) is shown. The expression patterns of GFP (A?D), 5-HT (A??D?) and merged images (A??D?) at 3 dpf (A), 4 dpf (B), 6 dpf (C) and 12 dpf (D) are shown. Green arrowheads: GFP + cells. Magenta arrowheads: 5-HT + cells. White arrowheads: GFP/5-HT double + cells. Anterior, to the left. The cells marked with 'e' in panels A? and B? are enterochromaffin cells. The data for Nos1 are presented as for 5-HT. The ratio of 5-HT+/GFP + cells in the GFP + cells and the ratio of Nos1+/GFP + cells in the GFP + cells did not change during the 3-dpf to 12-dpf stages and were 22%?32% for 5-HT and 37%?53% for Nos1. See the text for the details. Scale bar: 50 ?m.

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Gene expression patterns : GEP, 28, Nikaido, M., Izumi, S., Ohnuki, H., Takigawa, Y., Yamasu, K., Hatta, K., Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene, 12-21, Copyright (2018) with permission from Elsevier. Full text @ Gene Expr. Patterns