FIGURE

Fig. 2

ID

ZDB-FIG-180709-3

Publication

Dobrzycki et al., 2018 - An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation.

Other Figures

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Fig. 2

Runx1 mutants have significantly reduced levels of dnmt3bb.1 mRNA detected by ISH. (A) Representative images of ISH for dnmt3bb.1 in 33 hpf wild-type (blue), runx1^+/W84X (green) and runx1^W84X/W84X (orange) embryos, showing the expression in the dorsal aorta. The top panel indicates the location of the zoomed regions in a wild-type embryo. (B) 2% agarose gel showing representative genotypes of wild-type (WT), heterozygous (HET) and mutant (MUT) runx1 embryos, distinguished by RFLP. Yellow: wild-type 214 bp+124 bp bands, pink: 338 bp mutant band. First lane from the left: 100 bp DNA ladder. (C) Pixel intensity values of dnmt3bb.1 mRNA in runx1^W84X/W84X embryos (n=36) are significantly decreased compared to wild type (n=32) and heterozygotes (n=62) (ANOVA, P<0.001). The coefficients of variation are 24%, 22% and 21% for wild-type, heterozygote and mutant groups, respectively. Blue, green and orange data point correspond to the example images from panel A. The bars represent mean±s.d. ***P<0.001 (Games-Howell post-hoc test).

Expression Data

Gene:	dnmt3bb.1
Fish:	WT runx1^hg1/hg1 runx1^hg1/+
Anatomical Term:	ventral wall of dorsal aorta
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	runx1^hg1/hg1
Observed In:	ventral wall of dorsal aorta
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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