APOC2 mimetic peptide and VLDL rescue anemia in apoc2 mutants. a, b Representative images of BODIPY staining and quantitative results of BODIPY fluorescence intensity in WT, apoc2 mutants and the apoc2 mutants injected with APOC2 mimetic peptides (CII-a, active; CII-i, inactive) at 6.3 dpf (n = 5 in each group). c, d Representative images of blood cells in the caudal vein region and quantitative results of blood cell count in WT, apoc2 mutants and the apoc2 mutants injected with APOC2 mimetic peptides at 6.3 dpf (n = 6 in WT and mut + CII-i goups each; n = 8 in apoc2 mut group; n = 7 in mut + CII-a group). e In situ hybridization with cmyb/runx1 and β-globin probes in WT, apoc2 mutants and the apoc2 mutants injected with APOC2 mimetic peptides at 80 hpf. Embryos were injected with peptides at 2 dpf. f In situ hybridization with cmyb/runx1 and β-globin probes in WT, apoc2 and lpl mutants, including those injected with VLDL or LDL at 2 dpf. Embryos were analyzed at 80 hpf. Scale bars, 50 μm in a and c; 200 μm in e and f. Mean ± SEM; ***P < 0.001 (Student’s t test)
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