FIGURE

Fig. 5

ID

ZDB-FIG-180703-75

Publication

Knickmeyer et al., 2018 - TGFβ-facilitated optic fissure fusion and the role of bone morphogenetic protein antagonism

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Fig. 5

Timed induction of bmp4 affects expression of BMP antagonists. (a) Expression analysis of gremlin and follistatin by quantitative PCR, differential expression in heads of tg(hsp70:bmp4, cmlc2:GFP) embryos at 30 hpf after heat shock at 24 hpf as represented by the log2(fold change) of individual samples. Material from three individuals was pooled for one sample; n = 3, horizontal bars represent the arithmetic mean. p-Values for grem2b and fsta, 0.170 and 0.049, respectively. (b,c) WMISH for fsta (30 hpf) in tg(hsp70:bmp4, cmlc2:GFP) after heat shock at 24 hpf (b) and control embryos (c). Strong upregulation after the heat shock is seen in the optic fissure and other expression domains, but not the ciliary marginal zone (arrow). WMISHs in (b) and (c) were stained in parallel for the same amount of time.

Expression Data

Genes:	fsta grem2b
Fish:	AB af1Tg
Condition:	heat shock
Anatomical Terms:	head optic furrow retina ciliary marginal zone
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	af1Tg
Condition:	heat shock
Observed In:	head optic furrow
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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