Fig. 3

Surfen and oxalyl surfen rescue motoneuron defects and functional deficits in Tg[HuC::hTau^P301L; DsRed] embryos. a Immunohistochemical visualization of the synaptotagmin II protein (anti-znpl antibody) in 72 hpf wild-type (WT) and Tg[HuC::hTau^P301L; DsRed] (hTau^P301L) embryos treated for 2 days with 1% DMSO (hTau^P301L?+?1% DMSO), 80 mM LiCl (hTau^P301L?+?LiCl), 3 ?M surfen (hTau^P301L?+?surfen), 2 ?M oxalyl surfen (hTau^P301L?+?oxalyl surfen) or 3 ?M hemisurfen (hTau^P301L?+?hemisurfen), showed that surfen and oxalyl surfen markedly rescued the motorneuron axon defects seen in Tg[HuC::hTau^P301L; DsRed] embryos. The imaged caudal area corresponds to the box represented in (b). Scale bar: 50 ?m. c Quantification of the mean axonal branch number in the framed caudal area (b) of Tg[HuC::hTau^P301L; DsRed] embryos treated as in (a). The number of axonal branches was significantly increased in Tg[HuC::hTau^P301L; DsRed] embryos treated with surfen and oxalyl surfen when compared with their age-matched 1% DMSO siblings (n?=?10, ***P?<?0.001; **P?<?0.01; *P?<?0.05; ns: non-significant, Student?s t test). d Quantification of fluorescence staining intensity of znp1 labeling in the framed caudal area (b) of Tg[HuC::hTau^P301L; DsRed] embryos treated as in (a). Results showed that the fluorescence intensity of the znp1 staining was significantly increased in Tg[HuC::hTau^P301L; DsRed] embryos following surfen and oxalyl surfen treatments when compared with their age-matched 1% DMSO siblings (n?=?10, **P?<?0.01; *P?<?0.05, ns: non-significant, Student?s t test). e The touch evoked escape response was used to probe the motor behavior of Tg[HuC::hTau^P301L; DsRed] embryos treated for 1 day with 80 mM LiCl, 3 ?M surfen, 2 ?M oxalyl surfen or 3 ?M hemisurfen. The motor defect observed in Tg[HuC::hTauP301L; DsRed] embryos was significantly rescued following treatment with surfen and oxalyl surfen, but not hemisurfen (n?=?250, *P?<?0.05, ns: non-significant, Student?s t test)