FIGURE

Fig. 1

ID

ZDB-FIG-180627-49

Publication

Mukaigasa et al., 2018 - Nrf2 activation attenuates genetic endoplasmic reticulum stress induced by a mutation in the phosphomannomutase 2 gene in zebrafish

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Fig. 1

Up-regulated expression of Nrf2 target genes in it768 larvae that have splicing defects in the pmm2 gene. (A) Expression of gstp1 and hmox1a in WT or pmm2^it768 larvae injected or not with 1 pmol nrf2aMO. The closed and open arrowheads indicate the gills and liver, respectively. The numbers in each picture indicate the larvae exhibiting strong expression of gstp1 or hmox1a/tested larvae. (B) Nucleotide and deduced amino acid sequences of the major cDNA isolated from WT and pmm2^it768 larvae. The red “a” indicates the G-to-A mutation site of the it768 mutant in the zebrafish pmm2 genome. The green “R” indicates the amino acid residue corresponding to Arg141 in human PMM2. EcoRI indicates the EcoRI site only present in the WT cDNA.

Expression Data

Genes:	gstp1.2 hmox1a
Fish:	WT pmm2^it768/it768 pmm2^it768/it768 + MO1-nfe2l2a
Knockdown Reagent:	MO1-nfe2l2a
Anatomical Terms:	gill liver
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pmm2^it768/it768 pmm2^it768/it768 + MO1-nfe2l2a
Knockdown Reagent:	MO1-nfe2l2a
Observed In:	gill liver
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA