FIGURE

Fig. 2

ID

ZDB-FIG-180621-45

Publication

Wang et al., 2018 - Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish

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Fig. 2

MO-mediated Lpar2b knock-down reduced the number of NMs and hair cells in the posterior lateral line. (A,B) Epifluorescence images of 48 hpf Et(gata2:EGFP)^mp189b embryos injected with control or lpar2b MO. (C) Quantification of the number of trunk NMs (exclude terminal NMs) in one side of the pLL in (A,B). (D) Co-injection of MO-insensitive Lpar2b mRNA rescued the NMs phenotype caused by Lpar2b depletion. (E,F) Epifluorescence images of 72 hpf Tg(Brn3c:mGFP) embryos injected with control or lpar2b MO. (E′,F′) High-magnification images of hair cells in the L1 NMs in (E,F). (G) Quantification of the total number of hair cells in one side of the pLL (L1-L5) at 72 hpf. (H) Average number of hair cells per NM (L1-L5) at 72 hpf. (I,J) Snapshots from 6-h epifluorescence time-lapses movies of control or lpar2b MO-injected Tg(-8.0cldnb:lynEGFP) embryos at 30–36 hpf (Supplementary Movies 1, 2). (K) Quantification of the migration speed of the pLLP of (I,J). *p < 0.05; **p < 0.01; ^#p > 0.05 compared to control.

Expression Data

Gene:	EGFP
Fish:	s356tTg zf551Tg s356tTg + MO2-lpar2b zf551Tg + MO2-lpar2b
Knockdown Reagent:	MO2-lpar2b
Anatomical Terms:	posterior lateral line neuromast posterior lateral line neuromast hair cell
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s356tTg + MO2-lpar2b zf551Tg + MO2-lpar2b
Knockdown Reagent:	MO2-lpar2b
Observed In:	hair cell posterior lateral line neuromast whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.