FIGURE

Fig. 4

ID

ZDB-FIG-180621-20

Publication

Wopat et al., 2018 - Spine Patterning Is Guided by Segmentation of the Notochord Sheath

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Fig. 4

Activation of Notch Signaling in Alternating Sheath Domains Drives Notochord Segmentation

(A) At 3.7 mm SL, expression of entpd5a:pkRED and TP1:VenusPest, a reporter for Notch activation, shows that Notch signaling is active in double-positive and entpd5a⁺ domains during the formation of new segments (arrows).

(B) At 5.3 mm SL, expression of TP1:VenusPest persists in mature entpd5a⁺ segments.

(C) Formation of new entpd5a⁺ segments is halted upon treatment with 100 μM DAPT in 7 dpf larvae (DAPT treated, n = 9; DMSO treated, n = 6; p = 0.013) for 24 hr (see schematic; red dots indicate imaging time point). Control embryos treated with DMSO still form two or three segments (graph). Forty-eight hours following the washout of DAPT, entpd5a⁺ segment formation is recovered (arrows) in DAPT-treated fish. Scale bars for (A)–(C) are 100 μm.

(D) Photobleaching of a single TP1⁺ segment (red box) in a 4.0 mm larva shows that TP1 expression is significantly recovered by 4 hr (arrow).

(E) Recovery of the photobleached TP1⁺ segment (red box) is complete by 21 hr in DMSO but not in 100 μM DAPT. Scale bars for (D) and (E) are 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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