Fig. S1

In situ hybridization of acta1 and actc1 genes during zebrafish embryogenesis.

acta1a is expressed in the trunk skeletal muscle (arrows) at 24 hpf (A, A’), 48 hpf (B, B’) and 72 hpf (C, C’) and in the ocular muscle (o), developing pectoral fins (p), fin folds (f), hypaxial muscle (hy) and in the head musculature at 72 hpf (C, C’). acta1b is expressed in the heart (arrowheads) and trunk skeletal muscle (arrows) at 24 hpf (D, D’), 48 hpf (E, E’) and 72 hpf (F, F’). actc1a is expressed in the heart (arrowheads) and skeletal muscle (arrows) at 24 hpf (G, G’) and 48 hpf (H, H’), with expression in the skeletal muscle localized to the outer edges of the trunk muscle at 72 hpf (I, I’). actc1a is expressed in the ocular muscles (o), developing pectoral fins (p), fin folds (f), hypaxial muscle (h) and in the head musculature at 72 hpf (I, I’). actc1b is expressed in the heart (arrowheads) and trunk skeletal muscle (arrows) at 24 hpf (J, J’), 48 hpf (K, K’) and in the trunk skeletal muscle at 72 hpf (L, L’). actc1b is also expressed in the ocular muscles (o), developing pectoral fins (p), hypaxial muscle (h) and in the head musculature (h) at 72 hpf (L, L’).