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Fig. 1

ID
ZDB-FIG-180517-5
Publication
Zhang et al., 2017 - Leucine mediates autophagosome-lysosome fusion and improves sperm motility by activating the PI3K/Akt pathway
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Fig. 1

The effects of leucine treatment on zebrafish sperm motility the activation of PI3K/Akt signaling pathway. (A) Representative immunoblots showed p-Akt and Akt proteins after leucine treatment. Actin was used as the control. Relative protein level was shown from 3 independent experiments. (B) Quantification of the ratio of pAkt/Akt from 3 independent experiments. (C) The mRNA expression of PI3KR1 and PI3KCA were checked by qRT-PCR. (D) Comparisons of semen kinematic parameters between control group and leucine-treated group. Two kinematics parameters including progressive sperm and slow sperm were assessed with the computer-assisted sperm analysis (CASA) system. (E) Representative immunofluorescence pictures to detect mTOR and p-mTOR signals in control and leucine-treated zebrafish testes. Scale bar: 50 μm. Bars represent mean ± SEM of more than three separate experiments. *P < 0.05, **P < 0.01 and ***P < 0.001.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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