FIGURE

Fig. 4

ID

ZDB-FIG-180425-22

Publication

Hung et al., 2017 - A defect in the inner kinetochore protein CENPT causes a new syndrome of severe growth failure

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Fig. 4

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Fig. 4

Knock-down zebrafish model.

(A) Comparison of morpholino target effects at 24hpf and localization of morpholino targets in relation to the zebrafish cenpt gene and the conserved histone-fold domain (HFD, green box). (B) Effect of Mo8 (Mo8/8) on transcription of zebrafish cenpt in comparison to control morpholino (CoMo). (C) Dose response curve of Mo12 (Mo12/12) at 75hpf. On the bottom RT-PCR of Mo12 dose response curve. The upper band represents the wild-type band. (D) Head and eye measurements in Mo12 injected zebrafish at 48hpf. (E) Left panel shows a normal mitosis. The three right panels are examples of mitotic aberrations seen in Mo12 injected Tg(h2afv:GFP) zebrafish live imaging. (F) Time lapse of aberrant mitosis in Mo12 injected Tg(h2afv:GFP) zebrafish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cenpt WT + MO2-cenpt WT + MO3-cenpt kca66Tg; kca6Tg + MO3-cenpt
Knockdown Reagents:	MO1-cenpt MO2-cenpt MO3-cenpt
Observed In:	eye head mitotic chromosome movement towards spindle pole nuclear chromosome segregation whole organism
Stage Range:	1-cell to Long-pec

Phenotype Detail

Acknowledgments

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