FIGURE

Fig. 5

ID

ZDB-FIG-180420-8

Publication

Vierstraete et al., 2017 - Accurate quantification of homologous recombination in zebrafish: brca2 deficiency as a paradigm

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Fig. 5

Results of morpholino experiment. (a) Digital gel of RT-PCR (obtained by Labchip®GX software) of uninjected (MO−) and injected (MO+) embryos. Injecting the morpholino results in retention of intron 7, resulting in a transcript containing a premature stop codon. This is visualised as a longer RT-PCR fragment, using primers located in exon 4 and 8 (expected size: 250 bp; in case of intron 7 retention: 1600 bp). In the morphants, there is a clear band of 1600bp, indicative for intron retention. (b) Quantification of Rad51 foci in three morphants (MO+) and uninjected (MO−) embryos. Almost no Rad51 foci are present in the morphants. Error bars display 95% CI. (c) γH2AX foci are present in both irradiated wild types (upper left) and morphants (lower left). Wild types display clear Rad51 foci (upper right), while morphants display no Rad51 foci.

Expression Data

Antibodies:	Ab4-rad51 Ab10-h2afx
Fish:	WT WT + MO1-brca2
Condition:	x-ray
Knockdown Reagent:	MO1-brca2
Anatomical Term:	intestine cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-brca2
Condition:	x-ray
Knockdown Reagent:	MO1-brca2
Observed In:	intestine cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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