Fig. 6

ATF4 overexpression results in enhancement of ATs larval growth and adipocyte formation (A) Length comparison of ATs − Dox and WT-Dox larvae fed a HFD for two weeks. (32X magnification, scale bars: 200 μm). (B) Length comparison of ATs − Dox and WT-Dox larvae fed a low-fat diet (LFD) for two weeks (32X magnification, scale bars: 200 μm). (C) Length comparison of ATs − Dox larvae fed a HFD or LFD for two weeks (32X magnification, scale bars: 200 μm). (D) Average body weights of ATs − Dox and WT − Dox larvae fed a LFD or HFD (n = 40–50 for each groups). (E) Average body lengths of ATs − Dox and WT − Dox larvae fed a LFD or HFD (n = 40–50 for each groups). (F) ORO-stained visceral adipocytes in ATs − Dox compared to control zebrafish at 24 dpf (40X magnification, scale bars: 200 μm). Visceral adipocytes are circled. (G) The percentage of zebrafish larvae containing hyperplasia of visceral adipocytes (n = 40–50 for each groups). (H) ATF4 overexpression leads to adipocyte hyperplasia. H&E stain of the abdominal white adipose tissue (WAT) sections from 24 dpf ATs ± Dox and WT ± Dox fed a HFD for 2 weeks (400X magnification, scale bars: 10 μm). (I) The percentage of cell number in intra-abdominal fat pads of 24 dpf ATs ± Dox and WT ± Dox fed a HFD for 2 weeks (n = 10–15 for each groups). The asterisk represents statistically significant differences; *p < 0.01, and **p < 0.005.