FIGURE

Fig. 3

ID

ZDB-FIG-180418-18

Publication

Sarmah et al., 2017 - Embryonic Ethanol Exposure Affects Early- and Late-Added Cardiac Precursors and Produces Long-Lasting Heart Chamber Defects in Zebrafish

Other Figures

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Fig. 3

Myocardial differentiation was delayed in ethanol-exposed embryos. (A–C) In situ hybridization detecting nkx2.5 expression showed weak nkx2.5 expression in the linear heart tube (indicated fewer undifferentiated FHF derived cells) but strong expression at the outflow pole (indicated arrival of second heart field (SHF) progenitors) in the control embryo (A); E100 embryos displayed strong nkx2.5 expression in the linear heart tube but no expression at the outflow pole (B); E150 embryos showed strong nkx2.5 expression in the linear heart tube and at the outflow pole (C); (D–F) Strong MF20 antibody staining in the linear heart tube in control embryos labeled differentiated cardiomyocytes (D); MF 20 staining was weaker in the heart tube in ethanol-treated embryos (E,F); (G–I) Co-labeled images showed myocardial differentiation delay in ethanol-exposed embryos. White arrows: pointing to the outflow pole.

Expression Data

Gene:	nkx2.5
Antibody:	Ab-MF20
Fish:	WT
Condition:	chemical treatment by environment: ethanol
Anatomical Terms:	heart tube presumptive bulbus arteriosus
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by environment: ethanol
Observed In:	cardiac muscle cell myoblast differentiation heart tube presumptive bulbus arteriosus
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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