FIGURE

Fig. 3

ID

ZDB-FIG-180417-22

Publication

Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile

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Fig. 3

Lefty loss causes expanded expression of mesendodermal genes.

In situ hybridization comparing expression of endodermal (sox32/casanova) (A–E’’’) and mesodermal (noto/floating head) (F–J’’’) genes in wild type, lft1^-/- mutants, lft2^-/- mutants, and lft1^-/-;lft2^-/- mutants at the indicated developmental stages (dorsal views). Note upregulation of mesendoderm in lft1^-/-;lft2^-/- mutants starting at early gastrulation stages (e.g., shield stage). lft1^-/-;lft2^-/- embryos were generated from lft1^+/-;lft2^-/- incrosses, lft1^-/-;lft2^-/- incrosses, or lft1^-/-;lft2^-/- X lft1^+/-;lft2^-/- crosses. Embryos were genotyped after imaging (see Materials and methods for details).

Expression Data

Genes:	noto sox32
Fish:	WT lft1^a145/a145 lft2^a146/a146 lft2^a146/a146; lft1^a145/a145
Anatomical Terms:	endoderm mesoderm presumptive endoderm presumptive mesoderm
Stage Range:	Sphere to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lft2^a146/a146; lft1^a145/a145
Observed In:	endoderm mesoderm presumptive endoderm presumptive mesoderm
Stage Range:	Shield to 75%-epiboly

Phenotype Detail

Acknowledgments

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