FIGURE

Fig. 5

ID

ZDB-FIG-180308-24

Publication

Chen et al., 2017 - Imaging early embryonic calcium activity with GCaMP6s transgenic zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Excess Nodal signaling prolongs Ca²⁺ transient duration specifically in the DFCs. (A) Representative images of a single Ca²⁺ transient in a DFC at 6.5 hpf. (B) Quantification of Ca²⁺ transient duration in WT and Cyc/Ndr2-misexpressing embryos. Error bars represent standard deviation. ****, P≤0.0001. N=3 embryos in control and Cyc/Ndr2-misexpressing embryos, respectively. (C) Still images of Ca²⁺ transients in the DFCs of uninjected control and Cyc/Ndr2-misexpressing embryo in a time-lapse series. Arrowhead points to the DFC region that was imaged. (D) Quantification of DFC Ca²⁺ transient duration between DFCs and EVL cells in cyc/ndr2 RNA-injected embryos after CBX treatment. ****, P≤0.0001. N=2 embryos. (E) Quantification of DFC Ca²⁺ transient duration in Cyc/Ndr2-misexpressing embryos before and after CBX treatment. ns, not significant; N=4 embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 430(2), Chen, J., Xia, L., Bruchas, M.R., Solnica-Krezel, L., Imaging early embryonic calcium activity with GCaMP6s transgenic zebrafish, 385-396, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.