Fig. 3 S1

Rescue of krit1^ty219c mutant cardiovascular phenotypes by injection of mRNA encoding EGPF-Krit1.

(A–C) Projections of confocal z-stack images of (A) 48 hpf wild-type (WT), (B) krit1^ty219c mutant, and (C) rescued krit1^ty219c mutant hearts marked by Tg(kdrl:EGFP)^s843 expression. In comparison to WT, krit1^ty219c mutant hearts are dilated and lack the atrioventricular canal (AVC) (red arrowhead). (C) Injection of egfp-krit1 mRNA into krit1^ty219c mutants rescues the krit1^ty219c mutant cardiac phenotype (n = 82/82 krit1^ty219c mutant embryos rescued). (D–F) Projections of confocal z-stack images of 48 hpf caudal plexus regions of the vasculature marked by Tg(kdrl:EGFP)^s843 expression. (D) In comparison to the highly branched WT morphology of the caudal vein plexus (red asterisks), (E) the krit1^ty219c mutant vasculature has a fused morphology. (F) Injection of egfp-krit1 mRNA into krit1^ty219c mutants rescues the krit1^ty219c mutant vascular phenotype including a highly branched WT-like caudal vein plexus (red asterisks). A: atrium, V: ventricle. Scale bars are 50 µm.