FIGURE

Fig. S10

ID

ZDB-FIG-180202-8

Publication

Joris et al., 2017 - Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors

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Fig. S10

Sequential injection of RNA and sMOsrsf5a did not prevent partial rescue to occur. Based on developmental defect severity, sMOsrsf5a injected embryos were divided into three main classes; mild, intermediate and strong. Graphs representing the proportions of the three types of embryos after the indicated treatments. The total numbers of embryos pooled from n = 3 independent experiments are indicated above each bar. The graph shows mean+SEM, a Chi-square test with two degrees of freedom was performed, ***: P≤0.0001, NS: not significant. Coinjection of RNA and morpholino at one cell stage led to partial rescue. The same results were obtained when RNAs were injected at the one-cell stage and the sMOsrsf5a at the 4-cells stage in comparison to embryos injected at four cells stage with the sMOsrsf5a alone.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.