Fig. S3
Analysis of zygotic mutants at 24 hpf. (A-E) Bright-field lateral view of wildtype and ikk2-/- (zygotic) embryos obtained from heterozygotic parents with notochord defects at 24 hpf. A, wild-type embryos. B-E, ikk2-/- embryos classified into four categories based on severity of the notochord defects. Class I and II show notochord defects and Ushaped somites. Class III and IV show mild notochord defects and increased turbidity of the brain suggestive of apoptosis. (F-H) Lateral view of embryos stained by in situ hybridization using ntl probe. ikk2-/- embryos have either crooked notochord or its truncation. Scale bar: 250 μm. |