FIGURE

Fig. 4

ID

ZDB-FIG-180123-4

Publication

Chang et al., 2017 - Metformin Inhibits Cyst Formation in a Zebrafish Model of Polycystin-2 Deficiency

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Fig. 4

Metformin reduces leukocyte infiltration in pkd2 morphants. (A) Representative images of l-plastin-positive and mpx-positive cells surrounding the pronephric ducts in a control MO-injected embryo, a pkd2 morphant, and a pkd2 morphant treated with metformin (10 mM). Leukocytes were labelled using in situ hybridisation for l-plastin and mpx at 48 hpf. The lower panel shows an enlarged view of the dashed box indicated in the upper image. Scale bar, 200 µm. (B) Counts of the l-plastin-positive cells (within the region of yolk extension as indicated by the dashed box) in the controls and metformin-treated pkd2 morphants (2.5 mM to 20 mM). ***P < 0.001 compared with pkd2 morphants. Data represent two independent experiments, n = 7–11 per group. (C) Counts of the mpx-positive cells. **P < 0.01, ***P < 0.001 compared with pkd2 morphants. n.s. = not significant. Data represent two independent experiments, n = 16–20 per group.

Expression Data

Genes:	lcp1 mpx
Fish:	WT WT + MO3-pkd2
Condition:	chemical treatment by environment: metformin
Knockdown Reagent:	MO3-pkd2
Anatomical Terms:	macrophage monocyte neutrophil pronephric duct anatomical structure
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-pkd2
Condition:	chemical treatment by environment: metformin
Knockdown Reagent:	MO3-pkd2
Observed In:	leukocyte pronephric duct anatomical region pronephros morphogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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