FIGURE

Fig. 4

ID

ZDB-FIG-180105-4

Publication

Yang et al., 2017 - Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma

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Fig. 4

Effects of serotonin level and HSC activation on kras^V12-induced carcinogenesis. Three-month-old adult zebrafish were treated with dox with or without serotonin, BW23C86, PCPA, and SB204741 for 7 days. (A) Gross morphology and H&E staining of liver sections. (B) Quantification of tumor size as a percentage of total abdominal area. (C) Quantification of tumor histology observed in the H&E-stained liver sections of these zebrafish is shown on the right (n > 8 in each group). (D and E) IF staining and quantification of (D) PCNA+ and (E) caspase 3+ cells in liver sections of these zebrafish (n > 8). *P < .05. Scale bars: (A) 3 mm for gross observation images and 20 μm for histology images, and (D and E) 20 μm. DAPI, 4',6-diamidino-2-phenylindole.

Expression Data

Antibodies:	Ab1-casp3 Ab5-pcna
Fish:	WT gz32Tg
Conditions:	chemical treatment by environment: doxycycline chemical treatment by environment: 4-chloro-L-phenylalanine chemical treatment by environment: serotonin
Anatomical Terms:	female organism hematopoietic stem cell hematopoietic stem cell male organism hematopoietic stem cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gz32Tg
Conditions:	chemical treatment by environment: doxycycline chemical treatment by environment: 4-chloro-L-phenylalanine chemical treatment by environment: serotonin
Observed In:	female organism hematopoietic stem cell female organism liver liver neoplasm male organism hematopoietic stem cell male organism liver
Stage:	Adult

Phenotype Detail

Acknowledgments

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