Fig. 2

The Hippo pathway member yap1 regulates primordium size but not neuromast size.

(A–E) yap1 is not expressed in WT neuromasts (A) or neuromasts in which Notch (B and C) or Wnt signaling (D and E) are manipulated. (A'–E') yap1 is upregulated in the primordium after (B') Notch and (D') Wnt overexpression compared to (A') wildtype siblings and Wnt downregulation (E'). (C') Notch loss in mib1^ta52b mutants leads to downregulation of yap1. (F) Loss of yap1 by morpholino injections significantly reduces the number of cells in the leading 2/3 of WT, NICD and apc^mcr primordia, as well as the overall primordium size (H). (G) Notch and Wnt signaling significantly increases the number of cells in deposited L1 neuromasts, which is not rescued to a wildtype level by yap1 morpholino injections. Even though the size of NICD L1 and L2 pro-neuromasts is significantly reduced in yap1 morphants, they are still significantly larger than the corresponding WT neuromasts (F and G). Error bars represent standard error from one independent experiment (p<0.05=*, p<0.01=**, p<0.001=*** Student's t test). Scale bar is 25 μm.