FIGURE

Fig. 4

ID

ZDB-FIG-171120-23

Publication

Pollock et al., 2016 - Supervillin Is a Component of the Hair Cell's Cuticular Plate and the Head Plates of Organ of Corti Supporting Cells

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Fig. 4

Supervillin localizes to zebrafish hair cell CPs.

(A,B) Confocal micrographs of 4-dpf zebrafish anterior crista hair cells expressing GFP-fascin 2b (red) and labeled with anti-Svila (green). Arrowheads in A indicate hair cells out of focus or bent. Arrow indicates hair-bundle-localized GFP-fascin 2b. Asterisk indicates a CP. (C,D,E) Confocal micrographs of posterior macula hair cells from zebrafish expressing Acf7a-Citrine (red) and labeled with anti-Svila (green) (C,E) or phalloidin (green) (D). Asterisks in (C,D) indicate CPs. Acf7a-Citrine encircles the CP, localizes to the CP base, out of the focal plane in C-E, and is found weakly throughout the CP. (F) Schematic of the zebrafish posterior macula tissue with the location of Svila immunolabeling in green and Acf7a-Citrine indicated in red. Scale bars, 2 μm.

Expression Data

Genes:	EGFP mCherry svila
Fish:	macf1a^ct68aGt/+ cwr3Tg
Anatomical Terms:	anterior crista anterior macula hair cell inner ear posterior macula
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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