FIGURE

Fig. 4

ID

ZDB-FIG-171117-4

Publication

Lee et al., 2016 - Myc and Fgf Are Required for Zebrafish Neuromast Hair Cell Regeneration

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Fig. 4

Inhibition of Fgf signaling suppresses HC regeneration.

(A) A significant reduction in the number of HCs (HCS1⁺) regenerated after SU5402 treatment that was dose-dependent. Ctr, time-matched fish without neomycin treatment. (B) A significant reduction in the number of HCs regenerated in hsp70l:dn-fgfr1:GFP (Hsp) zebrafish neuromasts at 32°C or 37°C. AB: wild type AB fish. Ctr, time-matched transgenic fish without neomycin treatment. (C) A significant reduction in proliferation and transdifferentiation derived HCs after 20 μM SU5402 treatment for 72 hrs. ***p<0.001; **p<0.01. For all statistical analysis, n = 15 for each group. Three (for A) and two (for B,C) independent experiments were performed with similar results. (D) Fgf targets etv4 and spry4 were down-regulated by SU5402 (20 μM) in L1 neuromasts during HC regeneration. Scale bars: 10 μm.

Expression Data

Genes:	etv4 spry4
Fish:	AB
Conditions:	chemical ablation: neuromast hair cell, chemical treatment by environment: neomycin chemical treatment: SU5402, chemical treatment by environment: neomycin
Anatomical Term:	neuromast
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB pd1Tg
Conditions:	chemical ablation: neuromast hair cell, chemical treatment by environment: neomycin chemical treatment: SU5402, chemical treatment by environment: neomycin chemical treatment by environment: neomycin, heat shock
Observed In:	neuromast neuromast hair cell
Stage Range:	Day 6 to Days 7-13

Phenotype Detail

Acknowledgments

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