FIGURE

Fig. 5

ID

ZDB-FIG-171115-4

Publication

Lien et al., 2016 - Zebrafish cyclin Dx is required for development of motor neuron progenitors, and its expression is regulated by hypoxia-inducible factor 2α

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Fig. 5

The HIF2α transcription factor helps regulate ccndx gene expression and subsequent motor neuron development.

(A) MO knockdown of hif2a reduced the olig2 and isl1 signals in motor neurons (panels a, b, e, and f); co-injection of ccndx mRNA partially rescued this phenotype (panels c and g). Panels d and h: graphs showing the qPCR analysis confirmed the results of morphants with signals lower than control MO-injected embryos at 24 hpf. *P < 0.05. Scale bar, 100 mm. (B) A significantly higher proportion of hif2a morphant embryos had lower numbers of GFP-labeled primary motoneurons as compared to control MO-injected embryos (panels a and b) in the Tg:(isl1:GFP) transgenic line. The effect of rescue experiments with ccndx mRNA is shown in panel c. Quantitative data at 24 hpf are shown in panel d. (C) A significantly higher proportion of hif2a morphant embryos exhibited shorter CaP axons branches as compared to control MO-injected embryos (panels a and b). The effect of rescue experiments with ccndx mRNA is shown in panel c. Quantitative data at 24 hpf are shown in panel d. *P < 0.05.

Expression Data

Genes:	isl1a olig2
Fish:	WT + MO1-epas1b
Knockdown Reagent:	MO1-epas1b
Anatomical Term:	motor neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-epas1b rw0Tg + MO1-epas1b
Knockdown Reagent:	MO1-epas1b
Observed In:	CaP motoneuron axon motor neuron primary motor neuron
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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