Fig. 5

Expression patterns of ion transporters in pronephros of prl-deficient larvae.

(A–C) Whole mount in situ hybridization assay of atp1a1a.5 expression in pronephros of wild-type larvae (A), prl-deficient larvae (B) and prl-deficient larvae injected with prl mRNA (C) at 5 dpf in regular zebrafish egg water. (D–F) Whole mount in situ hybridization assay of solute carrier family 12, member 3 (slc12a3) expression in pronephros of wild-type larvae (D), prl-deficient larvae (E) and prl-deficient larvae injected with prl mRNA (F) at 5 dpf in regular zebrafish egg water. (G–I) Whole mount in situ hybridization assay of solute carrier family 9, subfamily A, member 3, tandem duplicate 2 (slc9a3.2) expression in pronephros of wild-type larvae (G), prl-deficient larvae(H) and prl-deficient larvae injected with prl mRNA (I) at 5 dpf in regular zebrafish egg water. Insets in A-I: the amplified images of the pronephros. (J) Expression levels of atp1a1a.5, slc12a3, and slc9a3.2 in decapitated larvae body samples from wild-type, prl-deficient larvae and prl-deficient larvae injected with prl mRNA at 5 dpf in regular zebrafish egg water assayed via qRT-PCR assay. (K) Expression levels of atp1a1a.5 in total tissue from wild-type and prl-deficient larvae at 5 dpf in regular zebrafish egg water assayed via qRT-PCR assay. *significant difference (P < 0.05). (a–c) different letters in two group mean significant difference (P < 0.05). Arrows, pronephric ducts; The qRT-PCR result shown here is the representative of the results obtained in two separate experiments. For in situ hybridization results, at least 12 embryos/genotype were analyzed in two separated experiments.