FIGURE

Fig. 3

ID

ZDB-FIG-171030-24

Publication

Prill et al., 2015 - Still Heart Encodes a Structural HMT, SMYD1b, with Chaperone-Like Function during Fast Muscle Sarcomere Assembly

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Fig. 3

SMYD1b is required for fast myosin incorporation during sarcomere assembly.

At 48hpf, fast myosin (F310—green) and actin (phalloidin—red) staining is visible in the premyofibrils in wt zebrafish tails (A&B), while absent from the premyofibrils in sth fast muscle tissue (C&D). Slow muscle (F59) develops normally in both wild type and still heart zebrafish at 48hpf (E&F, G&H). At 19hpf, in wild type embryos, fast myosin (F310) is beginning to be incorporated into the maturing myofibril (I) and overlaps (white arrowheads) with the developing actin (phalloidin) (J) fibers in trunk muscle (K, merge, K’ inset, white arrowhead). Fast myosin is not incorporated into the maturing premyofibril (L), although actin fibers are still present (M&N, N’ inset).

Expression Data

Antibodies:	Ab-F59 Ab-F310
Fish:	WT smyd1b^{tm123a/tm123a}
Anatomical Terms:	fast muscle cell skeletal muscle myofibril slow muscle cell
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smyd1b^{tm123a/tm123a}
Observed In:	fast muscle cell skeletal muscle myofibril sarcomerogenesis skeletal myofibril assembly
Stage Range:	20-25 somites to Long-pec

Phenotype Detail

Acknowledgments

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