FIGURE

Fig. 1

ID

ZDB-FIG-171030-10

Publication

Kotani et al., 2015 - Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213

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Fig. 1

Suppression of mysterin-α leads to the failure of hatching and motor deficits in zebrafish.

(a) Examination of the knockdown efficiency in morphants. Treatment with a low (1.7 ng) or high (5.1 ng) dose MO targeting mysterin-α (Mst) confirmed dose-dependent suppression of mysterin gene splicing at 2 dpf, as determined by RT-PCR. The lower band represents the products of impaired splicing (arrow). (b) Hatching defects in mysterin morphants. Morphants injected with a high dose of MO exhibit a significant hatching failure at 3 dpf. (c) Escape response to tactile stimulation at 60 hpf. Injection of a MO targeting mysterin reduces the movement of larvae in a dose-dependent manner. (d) Quantification of the swimming speed (mm/s) measured in 6 control morphants, 7 low-dose morphants, and 5 high-dose morphants at 60 hpf. **P < 0.01; ***P < 0.001.

Expression Data

Gene:	rnf213a
Fish:	WT WT + MO2-rnf213a
Knockdown Reagent:	MO2-rnf213a
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-rnf213a
Knockdown Reagent:	MO2-rnf213a
Observed In:	hatching swimming whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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