alkbh4 regulates actomyosin band formation in MZalkbh4 embryos. (A) Confocal images of phalloidin stained embryos at 50% epiboly, and phalloidin and anti-NMII double stained embryos at 75% epiboly. phalloidin and anti-NMII co-staining showing the defects of actomyosin band formation and marginal EVL cells morphology in MZalkbh4 embryos. The mutant embryos were collected not only at the same time point but also at the morphologically comparable stages compared with wild-type embryos. Embryos were laterally viewed with animal pole to the top and vegetal pole to the bottom. (B) Quantitative measurements of actomyosin band widths in (A) at 75% epiboly stage, which were represented by the widths of Actin and NMII staining in the E-YSL separately. (C) Quantitative measurements of the marginal EVL cell length/width ratios in (A) at both 50% and 75% epiboly. Ne, the number of observed embryos; Nc, the number of observed cells. *, p<0.01. (D) Epiboly defects caused by YSL-injection of alkbh4-MO2. At 4 hpf, 10 ng std-MO and alkbh4-MO2 were injected into the yolk cell of embryos and epiboly progression were observed at 50% and 80% epiboly stages. (E) Epiboly progression in (D) was measured by the average epiboly percentage of the embryos. Ne, the number of observed embryos. *, p<0.01. Scale bars: 50 μm in (A); 100 μm in (D).
|
