FIGURE

Fig. 1

ID

ZDB-FIG-170906-5

Publication

Kenyon et al., 2017 - Active nuclear transcriptome analysis reveals inflammasome-dependent mechanism for early neutrophil response to Mycobacterium marinum

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Fig. 1

Binary transgenic zebrafish model for regulatory profiling of neutrophils in response to M. marinum. (A) Driver line expressing BirA in neutrophils (i) is crossed with effector line ubiquitously expressing Avi-tagged Rangap (ii) for biotinylation of nuclei. In double transgenic fish, only the nuclei of neutrophils will be biotinylated (iii). (B) Effector line with ubiquitously expressed Avi-Cerulean-Rangap. (C) Neutrophil-specific BirA driver line with Citrine reporter. (D) Time course analysis of neutrophil infiltration of M. marinum. Embryos were injected with M. marinum at the 32-512-cell stage, fixed and immunofluorescence carried out for mpx protein at 2 dpi (i), 3 dpi (ii), 4 dpi (iii) and 5 dpi (iv). Neutrophil localisation is indicated by white arrows. (E) Neutrophil numbers in proximity to M. marinum were quantified at 2–5 dpi. Statistical significance was determined by one way ANOVA (n = 14–18, ****p < 0.0001). (F) Infection model for subsequent experiments.

Expression Data

Genes:	Cerulean Citrine
Antibody:	Ab4-mpx
Fish:	WT ct700aTg; ox121Tg
Condition:	bacterial treatment by injection: Mycobacterium marinum
Anatomical Terms:	neutrophil whole organism
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	bacterial treatment by injection: Mycobacterium marinum
Observed In:	neutrophil
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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