Fig. 1

Slc30a10 functions as a Mn exporter in zebrafish.

(A) In situ hybridization of wild-type zebrafish using the antisense slc30a10 probe, showing expression in the brain and liver (red arrows), as well as the YSL (red arrowheads). A sense probe was used as a negative control. (B) Semi-quantitative RT-PCR of slc30a10 mRNA in wild-type 1–7 dpf embryos, 14 dpf larvae, and 21 dpf larvae, showing the onset of expression at 5 dpf. (C) DNA and corresponding amino acid sequences of the wild-type (WT), 10-bp deletion (-10), and 1-bp insertion (+1) slc30a10 alleles following CRISPR/Cas9-based editing. (D) Summary of slc30a10 expression in WT and both slc30a10 mutant lines (n = 3 sets of 50 embryos/group). (E-G) Mn, Fe, and Zn concentration was measured in wild-type and mutant 1-week-old embryos (E; n = 6 sets of 1000 embryos/group), 3-week-old larvae (F; n = 3 sets of 50 larvae/group), and 4-month-old adults (G; n = 3 adults/group). (H-J) Heterozygous embryos were exposed to the indicated concentrations of Mn²⁺ (H), Fe³⁺ (ferric ammonium citrate, FAC; I), or Zn²⁺ (J) for 24 hours at 5 dpf. At 6 dpf, slc30a10 expression was measured; n = 3 sets of embryos/group. *p<0.05, **p<0.01, and ***p<0.001; N.S., not significant (p>0.05).