FIGURE

Fig. S8

ID

ZDB-FIG-170825-1

Publication

Tyrkalska et al., 2016 - Neutrophils mediate Salmonella Typhimurium clearance through the GBP4 inflammasome-dependent production of prostaglandins

Other Figures

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Fig. S8

Gbp4 regulates the number of neutrophils.

Zebrafish mpx:eGFP one-cell embryos were injected with standard control (Std) or Gbp4 MOs in combination with antisense (As), Gbp4 (A-E) or Gcsfa (C-E) mRNAs (A, D). Each dot represents the number of neutrophils from a single larva, while the mean ± SEM for each group is also shown. The sample size (n) is indicated for each treatment. (C) At 2 dpf, larvae were infected and survival determined as described in Figures 2A. (B, E) Representative images of green channels of whole larvae for the different treatments. Scale bars, 100 μm. The sample size for each treatment is shown in the graph in A and D, and is 290 in C . ns, not significant; ***p<0.001 according to log rank test (C) or ANOVA followed by Tukey multiple range test (A and D).

Expression Data

Gene:	EGFP
Fish:	i114Tg i114Tg + MO1-gbp4
Knockdown Reagent:	MO1-gbp4
Anatomical Term:	neutrophil
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-gbp4 i114Tg + MO1-gbp4
Condition:	bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium
Knockdown Reagent:	MO1-gbp4
Observed In:	neutrophil whole organism
Stage Range:	Long-pec to Days 7-13

Phenotype Detail

Acknowledgments

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