FIGURE

Fig. 13

ID

ZDB-FIG-170807-43

Publication

Murphy et al., 2017 - Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 13

Induction of ectopic cell death is not sufficient to disrupt eng2a expression in the anterior hindbrain of wildtype embryos.

TUNEL assay and in situ hybridization to eng2a RNA in wildtype embryos in which cell death is induced by Bcl2 inhibitor HA14–1. Wildtype DMSO (A,B,C) and HA14–1 (D,E,F) treated embryos were assessed for apoptotic nuclei by TUNEL assay followed by laser scanning confocal microscopy (brightfield A,D; darkfield B,E); and for eng2a expression by in situ hybridization (C,F). Embryos exhibit normal eng2a expression in the anterior hindbrain domain (C and F), regardless of the induction of higher levels of cell death by Bcl2 inhibitor treatment compared to controls (B versus E). Refer to F and for quantitative analysis.

Expression Data

Gene:	en2a
Fish:	AB
Condition:	chemical treatment: apoptosis inducer
Anatomical Term:	midbrain hindbrain boundary
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment: apoptosis inducer
Observed In:	central nervous system central nervous system cell death
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 146, Murphy, T., Melville, H., Fradkin, E., Bistany, G., Branigan, G., Olsen, K., Comstock, C.R., Hanby, H., Garbade, E., DiBenedetto, A.J., Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish, 10-30, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.