Fig. 5
- ID
- ZDB-FIG-170619-34
- Publication
- Lu et al., 2017 - MicroRNA-203a regulates fast muscle differentiation by targeting dmrt2a in zebrafish embryos
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miR-203a regulates muscle development in zebrafish embryos. (A) qRT-PCR detection of dmrt2a expression after miR-203a overexpression. (B) qRT-PCR detection of myhz-2 and myhz-5 expression level after miR-203a overexpression. NC, negative control mimic. (C) Whole mount in situ hybridization to check zebrafish muscle maker genes myhz-2 (a,b) and myhz-5 (c,d) in NC injected embryos (a,c) and miR-203a mimic injected embryos (b,d) at 24 hpf. Transverse sections of trunk regions in the corresponding embryos were shown in the right bottom. Error bars indicate mean ± SD, n = 3. 30 embryos for RT-PCR and 20 embryos for WISH in each experiment. Student's t-test was used for statistical analysis (*P < 0.05). |
Reprinted from Gene, 625, Lu, C., Wu, J., Xiong, S., Zhang, X., Zhang, J., Mei, J., MicroRNA-203a regulates fast muscle differentiation by targeting dmrt2a in zebrafish embryos, 49-54, Copyright (2017) with permission from Elsevier. Full text @ Gene