Fig. 9
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- ZDB-FIG-170609-14
- Publication
- Kelu et al., 2017 - Ca2+ release via two-pore channel type 2 (TPC2) is required for slow muscle cell myofibrillogenesis and myotomal patterning in intact zebrafish embryos.
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Visualization of TPC2, IP3R (type III) and ryanodine receptors (RyR) by dual-immunolabeling and dual-colour stimulated emission depletion (STED) super resolution imaging. Representative examples of muscle cells that were dissociated from the trunk of zebrafish embryos at 48 hpf, plated onto a coverslip for 1 h and then fixed and dual-immunolabeled with either (A-C,C*) the 2137A anti-TPC2 and 34C anti-ryanodine receptor primary antibodies or (D-F,F*) the IP3R (type III) and 34C antibodies. STED images of: (A) TPC2, (B) RyR, and (C) the TPC2 and RyR images when merged, as well as (D) IP3R (type III), (E) RyR and (F) IP3R and RyR images when merged. The regions bounded by the white squares in panels C and F are shown at higher magnification in panels C* and F*. The white lines in panel C* indicate the presence of distinct gaps between the RyR and TPC2 clusters when observed via STED super-resolution imaging. Z and M indicate the Z- and M-lines of the sarcomere, respectively. Scale bars, 2 µm (A-F); and 200 nm (C*,F*). (G) Schematic illustration of two sarcomeres and the relative localization of TPC2 and RyR in the adjacent lysosomes and SR, respectively (see Fig. 10 for a 3D representation of an SMC). |
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Reprinted from Developmental Biology, 425(2), Kelu, J.J., Webb, S.E., Parrington, J., Galione, A., Miller, A.L., Ca2+ release via two-pore channel type 2 (TPC2) is required for slow muscle cell myofibrillogenesis and myotomal patterning in intact zebrafish embryos., 109-129, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.